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Recognizing these limitations, clinical practice has increasingly shifted away from restricted SNP panels toward what is widely considered one of the most cost-effective strategies to date: whole-exome sequencing (WES

Cell lines and cell culture Human embryonic kidney 293 (HEK293, #CRL-1573) cells were obtained from ATCC and were cultured in DMEM/F-12 (Dulbeccos Modified Eagle Medium/Nutrient Mixture F-12, Gibco #11330-032) medium with 10% fetal bovine serum (FBS, Sigma-Aldrich #F2442) and 1x penicillin streptomycin (Corning #30-002 CL) at 37 C in a 5% CO2 incubator

COPD creates persistent airflow limitation, mucus buildup, oxidative stress, and inflammation in the airway